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. 1974 Apr;61(1):188-200.
doi: 10.1083/jcb.61.1.188.

Analytical study of microsomes and isolated subcellular membranes from rat liver. I. Biochemical methods

H Beaufay, A Amar-Costesec, E Feytmans, D Thinès-Sempoux, M Wibo, M Robbi, J Berthet

Analytical study of microsomes and isolated subcellular membranes from rat liver. I. Biochemical methods

H Beaufay et al. J Cell Biol. 1974 Apr.

Abstract

The series introduced by this paper reports the results of a detailed analysis of the microsomal fraction from rat liver by density gradient centrifugation. The biochemical methods used throughout this work for the determination of monoamine oxidase, NADH cytochrome c reductase, NADPH cytochrome c reductase, cytochrome oxidase, catalase, aminopyrine demethylase, cytochromes b(5) and P 450, glucuronyltransferase, galactosyltransferase, esterase, alkaline and acid phosphatases, 5'-nucleotidase, glucose 6-phosphatase, alkaline phosphodiesterase I, N-acetyl-beta-glucosaminidase, beta-glucuronidase, nucleoside diphosphatase, aldolase, fumarase, glutamine synthetase, protein, phospholipid, cholesterol, and RNA are described and justified when necessary.

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