doi: 10.1126/science.415360.
Covalent immunoglobulin assembly in vitro: reactivity of light chain covalent dimers (L2) and blocked light chain monomers
- PMID: 415360
- DOI: 10.1126/science.415360
Item in Clipboard
Covalent immunoglobulin assembly in vitro: reactivity of light chain covalent dimers (L2) and blocked light chain monomers
Science.
.
Abstract
Covalent light chain dimers (L2) and cysteine-blocked L chain monomers readily react with partially reduced heavy (H) chains. A rapid disappearance of these blocked L chain species is followed by the appearance of covalent intermediates-HL, H2, and H2L-leading to fully assembled H2L2. The mechanism of initial disulfide bond formation between heavy and light chains is disulfide interchange.
Similar articles
-
Acquisition of the covalent quaternary structure of an immunoglobulin G molecule. Reoxidative assembly in vitro.Biochemistry. 1977 May 3;16(9):2016-25. doi: 10.1021/bi00628a040. Biochemistry. 1977. PMID: 403936
-
Acquisition of the covalent quaternary structure of an immunoglobulin G molecule. Theoretical reoxidation models.Biochemistry. 1977 May 3;16(9):2026-31. doi: 10.1021/bi00628a041. Biochemistry. 1977. PMID: 403937
-
A kinetic study in vitro of the reoxidation of interchain disulfide bonds in a human immunoglobulin IgGLk. Correlation between sulfhydryl disappearance and intermediates in covalent assembly of H2L2.Proc Natl Acad Sci U S A. 1975 Jan;72(1):353-7. doi: 10.1073/pnas.72.1.353. Proc Natl Acad Sci U S A. 1975. PMID: 235127 Free PMC article.
-
Immunoglobulin structure, synthesis, secretion, and relation to neoplasms of B cells.Clin Haematol. 1977 Jun;6(2):503-32. Clin Haematol. 1977. PMID: 410572 Review. No abstract available.
-
Bence-Jones proteins and light chains of immunoglobulins (first of two parts).N Engl J Med. 1976 Jan 1;294(1):17-23. doi: 10.1056/NEJM197601012940105. N Engl J Med. 1976. PMID: 53785 Review. No abstract available.
Cited by
-
Self-association of human immunoglobulin kappa I light chains: role of the third hypervariable region.Proc Natl Acad Sci U S A. 1980 Feb;77(2):1144-8. doi: 10.1073/pnas.77.2.1144. Proc Natl Acad Sci U S A. 1980. PMID: 6767243 Free PMC article.
-
Alternative pathways of disulfide bond formation yield secretion-competent, stable and functional immunoglobulins.Mol Immunol. 2008 Nov;46(1):97-105. doi: 10.1016/j.molimm.2008.07.005. Epub 2008 Aug 9. Mol Immunol. 2008. PMID: 18692901 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
