Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1974 Dec;71(12):4843-6.
doi: 10.1073/pnas.71.12.4843.

Heterogeneity of membrane vesicles from Escherichia coli and their subfractionation with antibody to ATPase

J F Hare, K Olden, E P Kennedy

Heterogeneity of membrane vesicles from Escherichia coli and their subfractionation with antibody to ATPase

J F Hare et al. Proc Natl Acad Sci U S A. 1974 Dec.

Abstract

The energy-transducing, Mg-Ca activated ATPase (ATP phosphohydrolase, EC 3.6.1.3) of E. coli is located on the inner surface of the cytoplasmic membrane. Antibody to purified ATPase has now been used to demonstrate that membrane vesicles as ordinarily prepared by the lysozyme-EDTA method consist of two distinct populations. About half the vesicles are everted, and thus readily agglutinated by antibody to ATPase, while half are right-side out. NADH oxidase (reduced NAD:O(2) oxidoreductase EC 1.6.99.3) activity is associated almost entirely with everted vesicles, while the ability to concentrate proline is a property of the right-side out vesicles. The results explain the failure of previous workers to observe the energization of membrane vesicles by oxidation of NADH.

PubMed Disclaimer

References

    1. J Biol Chem. 1971 Sep 10;246(17):5518-22 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2663-7 - PubMed
    1. J Bacteriol. 1973 May;114(2):772-81 - PubMed
    1. Biochim Biophys Acta. 1972 Aug 4;265(3):367-416 - PubMed
    1. Biochim Biophys Acta. 1973 Oct 25;323(3):429-40 - PubMed

Publication types