Comparison of synchronization of primate circadian rhythms by light and food

Abstract

Several circadian rhythms in squirrel monkeys (Saimiri sciureus) entrained by two different agents were studied to compare their mode of coupling with the environmental zeitgebers. Synchronization was accomplished either by light-dark cycles consisting of 12 h of 600 lx followed by 12 h of less than 1 lx (LD 12:12), or by eat-fast cycles in which the animals could eat for 3 h and then had to fast for the remaining 21 h each day (EF 3:21). The rhythms of drinking, colonic temperature, and urinary potassium and water excretion were measured in chair-acclimatized monkeys. The drinking and urinary rhythms were more reproducible (smaller mean variance) and more stable (smaller standard deviation of the timing of a phase reference point) in EF than in LD cycles, whereas the temperature rhythm was more tightly controlled by LD cycles than by EF cycles. In constant light an 8-h phase delay in the EF cycle caused the drinking and urinary rhythms to resynchronize to the EF cycle within one day, while the temperature rhythm required about 6 days to resynchronize. In contrast, previously published data for a similar phase delay in the LD cycle with food available ad libitum show that the drinking and temperature rhythms resynchronized more rapidly than the urinary rhythms. These results indicate that separate mechanisms are involved in transducing temporal cues from LD and EF cycles in the circadian timekeeping system of these nonhuman primates.