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. 2026 Jan 23.
doi: 10.1038/s41598-025-31170-3. Online ahead of print.

Curcumin attenuates the progression of hemorrhoids through the inhibition of angiogenesis via miR-190a-5p/TGFBR2

Heng Zhang  1 , Chunling Li  2 , Qingsheng Liu  2 , Weiping Wan  2 , Jian Cheng  2 , Xu Han  2
Affiliations

Curcumin attenuates the progression of hemorrhoids through the inhibition of angiogenesis via miR-190a-5p/TGFBR2

Heng Zhang et al. Sci Rep. .

Abstract

Hemorrhoids are common diseases of the anorectal system and are usually accompanied by vascular proliferation and edema. Curcumin is a natural molecule with potential anti-inflammatory, antitumor and antioxidant effects. This study aimed to explore the effect and molecular mechanism of curcumin in alleviating angiogenesis in rat hemorrhoids. A rat model of hemorrhoids was established via glacial acetic acid induction. Human venous endothelial cells were collected to perform cell experiments. The rats received curcumin at 25, 50, or 100 mg/kg/d. The rats were scored for perianal symptoms and inflammation levels, and the expression of angiogenesis-related factors was evaluated by immunofluorescence. Bioinformatics analysis, dual-luciferase, RT‒qPCR and Western blotting were used to verify the molecular mechanism of curcumin in the treatment of hemorrhoids. This study demonstrates that curcumin inhibited angiogenesis in endothelial cells and relieved the symptoms of hemorrhoids in rats. Notably, the expression of miR-190a-5p was significantly downregulated in hemorrhoids, whereas curcumin promoted the expression of miR-190a-5p. The results also revealed that miR-190a-5p could inhibit angiogenesis and inflammation in endothelial cells by targeting TGFBR2, thereby alleviating the development of hemorrhoids. Mechanistically, curcumin inhibits the expression of TGFBR2 by upregulating miR-190a-5p, effectively blocking the angiogenesis ability of endothelial cells and thereby attenuating the progression and development of hemorrhoids in rats. Curcumin effectively inhibits angiogenesis in endothelial cells and relieves inflammation and the progression of hemorrhoids by regulating the miR-190a-5p/TGFBR2 molecular pathway.

Keywords: Angiogenesis; Curcumin; Hemorrhoids; Inflammation; TGFBR2; miR-190a-5p.

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Conflict of interest statement

Declarations. Competing interests: The authors declare no competing interests. Ethical approval: All animal experiments complied with the ARRIVE guidelines and were carried out in accordance with the National Research Council’s Guide for the Care and Use of Laboratory Animals. All animal experimental protocols were approved by the Ethics Review Committee of Animal Experiments of Kunming Medical University (kmmu20211591). This study was officially approved by the Medical Ethics Committee of Kunming Municipal Hospital of Traditional Chinese Medicine (No.: [2021] Lunbei Zi (21)). Informed consent to be included in the study was obtained from all patients.

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