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. 1966 Jun 17;152(3729):1631-4.
doi: 10.1126/science.152.3729.1631.

Staining tissue for light and electron microscopy by bridging metals with multidentate ligands

Staining tissue for light and electron microscopy by bridging metals with multidentate ligands

J S Hanker et al. Science. .

Abstract

"Osmium black," a pigment very useful for cytological staining in both light and electron microscopy, may be deposited selectively at the tissue-binding sites of other metal ions by bridging OsO(4) to the tissue bound metal ion through a multidentate ligand.

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