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. 1966 May;99(2):356-66.
doi: 10.1042/bj0990356.

The N- and c-terminal amino acid sequences of the heavy chain from a pathological human immunoglobulin IgG

E M Press, P J Piggot, R R Porter

The N- and c-terminal amino acid sequences of the heavy chain from a pathological human immunoglobulin IgG

E M Press et al. Biochem J. 1966 May.

Abstract

The heavy chain of a pathological human immunoglobulin IgG and also the Fd fragment have been isolated. No free alpha-amino group was present on either and the N-terminal sequence of both has been identified as pyrrolid-2-one-5-carbonylvalylthreonine. Splitting at the four methionine residues of the heavy chain with cyanogen bromide gave five fractions. The fraction from the C-terminal end of the chain was isolated in high yield and the amino acid sequence was: His-Glu-Ala-Leu-His-Asp(NH(2))-His-Tyr-Thr-Glu(NH(2))-Lys-Ser-Leu-Ser-Leu-Ser-Pro-Gly These results give strong support to the view that the heavy chain of immunoglobulin is a single peptide chain.

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References

    1. J Biol Chem. 1960 Mar;235:633-47 - PubMed
    1. J Biol Chem. 1962 Jun;237:1845-50 - PubMed
    1. J Biol Chem. 1949 Dec;181(2):719-29 - PubMed
    1. J Biol Chem. 1956 Apr;219(2):623-42 - PubMed
    1. Biochemistry. 1965 Aug;4(8):1473-8 - PubMed

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