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. 1978 Feb;104(2):187-91.
doi: 10.1099/00221287-104-2-187.

Studies on the chemical composition of lipopolysaccharide from Neisseria meningitidis group B

G A Limjuco, Y D Karkhanis, J Y Zeltner, R Z Maigetter, J J King, D J Carlo

Studies on the chemical composition of lipopolysaccharide from Neisseria meningitidis group B

G A Limjuco et al. J Gen Microbiol. 1978 Feb.

Abstract

A lipopolysaccharide was isolated from Neisseria meningitidis group B by phenol/water extraction and purified by differential ultracentrifugation. This preparation exhibited endotoxic properties as shown by the limulus-lysate assay. Mild acid hydrolysis of the lipopolysaccharides yielded a lipid A fraction and a polysaccharide fraction. The lipid A fraction contained fatty acids, phosphorus and glucosamine. Analysis of the polysaccharide fraction revealed the presence of glucose, galactose, glucosamine, 2-keto-3-deoxyoctonic acid and phosphorus. There was no heptose.

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