Electrophoretic studies of sera from cattle vaccinated or naturally infected with Brucella abortus

Abstract

Examinations of the electrophoretic behaviour in cellulose acetate were made on the proteins of sera from 18 field cases of brucellosis, 15 calves recently vaccinated with strain 19 Brucella abortus and from 28 normal cattle. The total protein and gamma-globulin content of the sera of the naturally infected, serologically positive cattle tended to be somewhat higher than that of sera from most normal cattle. A few of the serologically-negative normals also had defenitely elevated serum gamma-globulin values. In the vaccinated calves there was an increase both in total serum protein and gamma-globulin but its magnitude in different individuals was not directly related to the rise in their antibody titre.