Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1967 Aug;104(2):616-26.
doi: 10.1042/bj1040616.

Cyanogen bromide cleavage and partial sequence of the heavy chain of a pathological immunoglobulin G

P J Piggot, E M Press

Cyanogen bromide cleavage and partial sequence of the heavy chain of a pathological immunoglobulin G

P J Piggot et al. Biochem J. 1967 Aug.

Abstract

The heavy chain of a pathological immunoglobulin G (Daw) of type L, subclass gamma(2b) (We) and Gm(a+)(f-), has been cleaved with cyanogen bromide. The five fragments resulting from the cleavage have been isolated and analysed. The papain-digest fragments, Fab and Fc, have also been cleaved with cyanogen bromide and the products analysed and compared with those from the heavy chain. The order of the five fragments in the heavy chain has been established and the location of some of the inter-chain and inter-fragment disulphide bonds has been determined. The sequence of the N-terminal fragment consisting of 34 residues is reported.

PubMed Disclaimer

References

    1. Proc R Soc Lond B Biol Sci. 1966 Nov 22;166(1003):159-75 - PubMed
    1. Arch Biochem Biophys. 1962 Sep;Suppl 1:174-80 - PubMed
    1. J Biol Chem. 1959 Jul;234(7):1761-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1964 Oct;52:1099-106 - PubMed
    1. Biochem J. 1965 Oct;97(1):32-6 - PubMed