Studies on transmissible gastroenteritis of swine. 3. The effect of selective inhibitors of viral replication on a cytopathogenic virus from transmissible gastroenteritis

Abstract

The effect on the plaque production of the Purdue strain of cytopathogenic virus from transmissible gastroenteritis of swine by 5-bromo-2'-deoxyuridine (BUDR), 5-iodo-2'-deoxyuridine (IUDR), actinomycin-D, puromycin, and amantadine-HCI (Symmetral) has been studied.Amantadine-HCI reduced the plaque-forming units of virus per ml by approximately 98%. Puromycin prevented almost all virus reproduction while actinomycin-D caused approximately a 22% reduction. Both IUDR and BUDR produced approximately a 20% increase in plaque-forming units of virus per ml. Swine testis cells stained with acridine orange early in the course of infection contained brick-red particles in the cytoplasm, indicative of a ribonucleic acid (RNA) type virus.