doi: 10.1007/BF00495757.
An efficient method to produce specific anti-actin
- PMID: 417050
- DOI: 10.1007/BF00495757
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An efficient method to produce specific anti-actin
Histochemistry.
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Abstract
The production of affinity column purified (specific) anti-actin is described. With the immunization scheme employed, all rabbits produced precipitating antibodies over several months, so that 30 mg specific anti-actin per rabbit could be isolated in 6 months. The antibodies against native and detergent denatured smooth muscle actin are characterized by immunodiffusion tests, staining of the I-band of isolated myofibrils and stress fibers in tissue culture cells, using indirect immunofluorescence.
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