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. 1978 Apr 4;55(3):177-84.
doi: 10.1007/BF00495757.

An efficient method to produce specific anti-actin

B M Jockusch, K H Kelley, R K Meyer, M M Burger

An efficient method to produce specific anti-actin

B M Jockusch et al. Histochemistry. .

Abstract

The production of affinity column purified (specific) anti-actin is described. With the immunization scheme employed, all rabbits produced precipitating antibodies over several months, so that 30 mg specific anti-actin per rabbit could be isolated in 6 months. The antibodies against native and detergent denatured smooth muscle actin are characterized by immunodiffusion tests, staining of the I-band of isolated myofibrils and stress fibers in tissue culture cells, using indirect immunofluorescence.

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