doi: 10.1007/BF00495762.
Histochemical quantification of dopa-oxidase (tyrosinase) activity in epidermal melanocytes
- PMID: 417051
- DOI: 10.1007/BF00495762
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Histochemical quantification of dopa-oxidase (tyrosinase) activity in epidermal melanocytes
Histochemistry.
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Abstract
The graphical method of Lineweaver-Burk was applied to the histochemical system to obtain quantitative information on the dopa-oxidase activity in human epidermal melanocytes. In vitro experiment showed that the incubation time necessary for yielding a certain amount of dopa-melanin is related to the amount of enzyme present. In the case of histochemical experiment, the time required for the first appearance of dopa-melanin deposition in melanocytes at various substrate concentrations was plotted against the reciprocal of the substrate concentration. This technique made it possible to compare the dopa-oxidase activity at various sites of the tissue.
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