Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Apr 4;55(3):235-43.
doi: 10.1007/BF00495762.

Histochemical quantification of dopa-oxidase (tyrosinase) activity in epidermal melanocytes

Y Sasai, S Kumano, M Takahashi, H Nakano

Histochemical quantification of dopa-oxidase (tyrosinase) activity in epidermal melanocytes

Y Sasai et al. Histochemistry. .

Abstract

The graphical method of Lineweaver-Burk was applied to the histochemical system to obtain quantitative information on the dopa-oxidase activity in human epidermal melanocytes. In vitro experiment showed that the incubation time necessary for yielding a certain amount of dopa-melanin is related to the amount of enzyme present. In the case of histochemical experiment, the time required for the first appearance of dopa-melanin deposition in melanocytes at various substrate concentrations was plotted against the reciprocal of the substrate concentration. This technique made it possible to compare the dopa-oxidase activity at various sites of the tissue.

PubMed Disclaimer

References

    1. J Invest Dermatol. 1966 Aug;47(1):35-8 - PubMed
    1. J Invest Dermatol. 1956 Apr;26(4):235-7 - PubMed
    1. Acta Derm Venereol. 1968;48(3):175-80 - PubMed
    1. Acta Derm Venereol. 1971;51(2):93-7 - PubMed
    1. J Invest Dermatol. 1966 Oct;47(4):293-5 - PubMed