Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Feb 28;4(1):45-50.
doi: 10.1007/BF00775180.

The effect of various conditions of chromatin isolation on the nucleosomal structure of the isolated chromatin

V I Popenko, Y Y Vengerov

The effect of various conditions of chromatin isolation on the nucleosomal structure of the isolated chromatin

V I Popenko et al. Mol Biol Rep. .

Abstract

Chromatin from calf thymus isolated under hypotonic conditions in the presence of various agents was investigated by methods of electron microscopy prior to and after EDTA treatment. It is shown that the presence of chelating agents and, especially, the application of considerable mechanical forces in the course of isolation may cause damage to the nucleosomal structure of the chromatin. Moreover, sufficiently great mechanical forces are liable to destroy the structure of the chromatin nucleosomal fibres even when they are packed in structures of a higher order of organization of the chromatin.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Dokl Akad Nauk SSSR. 1977;232(6):1442-4 - PubMed
    1. Exp Cell Res. 1976 Jan;97:111-9 - PubMed
    1. Science. 1975 Mar 28;187(4182):1203-6 - PubMed
    1. Annu Rev Biochem. 1973;42:355-78 - PubMed
    1. Exp Cell Res. 1976 Jul;100(2):223-7 - PubMed

MeSH terms