doi: 10.1042/bj1690697.
Demineralization in organic solvents by alkylammonium salts of ethylenediaminetetra-acetic acid
- PMID: 417721
- PMCID: PMC1183843
- DOI: 10.1042/bj1690697
Item in Clipboard
Demineralization in organic solvents by alkylammonium salts of ethylenediaminetetra-acetic acid
Biochem J.
.
Abstract
1. Insoluble salts of alklaine earth metals (e. g. calcium phosphate) dissolve in non-aqueous solvents containing EDTA salts of organic cations (e.g. tetraethylammonium). 2. Bone is efficiently demineralized in ethanolic trimethylammonium EDTA. Retention in the matrix of water-soluble materials (e.g. proteoglycan) is much better than in standard aqueous EDTA demineralization procedures.
Similar articles
-
Biochemical and immuno- and lectin-histochemical studies of solubility and retention of bone matrix proteins during EDTA demineralization.Histochem J. 1992 Feb;24(2):78-85. doi: 10.1007/BF01082443. Histochem J. 1992. PMID: 1577625
-
Effects of demineralization in an ethanolic solution of triethylammonium EDTA on solubility of bone matrix components and on ultrastructural preservation.Calcif Tissue Int. 1980;32(2):175-9. doi: 10.1007/BF02408537. Calcif Tissue Int. 1980. PMID: 6773635
-
Sialic acids in bone demineralised in organic solvents.Basic Appl Histochem. 1980;24(1):17-21. Basic Appl Histochem. 1980. PMID: 6246870
-
Staining of demineralized cartilage. I. Alcoholic versus aqueous demineralization at neutral and acidic pH.Histochemistry. 1981 Dec;73(3):385-90. doi: 10.1007/BF00495653. Histochemistry. 1981. PMID: 6173354
-
Recent Advances in Solvents for the Dissolution, Shaping and Derivatization of Cellulose: Quaternary Ammonium Electrolytes and their Solutions in Water and Molecular Solvents.Molecules. 2018 Feb 25;23(3):511. doi: 10.3390/molecules23030511. Molecules. 2018. PMID: 29495344 Free PMC article. Review.
Cited by
-
Biochemical and immuno- and lectin-histochemical studies of solubility and retention of bone matrix proteins during EDTA demineralization.Histochem J. 1992 Feb;24(2):78-85. doi: 10.1007/BF01082443. Histochem J. 1992. PMID: 1577625
-
Proteoglycan-fibrillar collagen interactions.Biochem J. 1988 Jun 1;252(2):313-23. doi: 10.1042/bj2520313. Biochem J. 1988. PMID: 3046606 Free PMC article. Review. No abstract available.
-
Incorporation of (35S)sulfate and (3H)glucosamine into glycoaminoglycans in rat incisor predentine and dentine: comparison by autoradiography of fixation by rapid-freezing, freeze-substitution, and aldehyde fixation.Calcif Tissue Int. 1985 Sep;37(5):511-8. doi: 10.1007/BF02557834. Calcif Tissue Int. 1985. PMID: 3933790
-
Ultrastructural and biochemical observations on proteoglycans and collagen in the mutable connective tissue of the feather star Antedon bifida (Echinodermata, Crinoidea).J Anat. 1993 Aug;183 ( Pt 1)(Pt 1):1-11. J Anat. 1993. PMID: 8270464 Free PMC article.
-
Use of histological samples for assessing skeletal calcium.J Clin Pathol. 1987 Jan;40(1):50-2. doi: 10.1136/jcp.40.1.50. J Clin Pathol. 1987. PMID: 3546389 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
