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. 1967 Sep;20(5):720-3.
doi: 10.1136/jcp.20.5.720.

Developments in immunofluorescence: the need for standardization

G D Johnson, E H Beutner, E J Holborow

Developments in immunofluorescence: the need for standardization

G D Johnson et al. J Clin Pathol. 1967 Sep.

Abstract

DIFFICULTIES EXPERIENCED BY THE NEWCOMER TO THE FLUORESCENT ANTIBODY STAINING METHOD LARGELY ARISE FROM TWO CAUSES: (1) the many modifications in the procedure which have been suggested over the last decade; (2) the variability of the basic reagent, fluorescent conjugate, in terms of potency and specificity. The ensuing problem of non-reproducibility is clearly an important factor for clinical laboratories now faced with increasing demands for routine tests involving immunofluorescence. In this paper a simple approach to standardization is described which, if adopted, would lead to a more efficient use of the method.

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References

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