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. 1968 Nov;96(5):1500-6.
doi: 10.1128/jb.96.5.1500-1506.1968.

Reduction of nonspecific background staining in the fluorescent treponemal antibody-absorption test

M E Roberts, J N Miller, G F Binnings

Reduction of nonspecific background staining in the fluorescent treponemal antibody-absorption test

M E Roberts et al. J Bacteriol. 1968 Nov.

Abstract

The nonspecific background fluorescence which occurs with the fluorescent treponemal antibody-absorption test for syphilis was found to result from a reaction between serum-treated Treponema pallidum organisms and the conjugated antihuman gamma-globulin. It was also shown that beta-lipoprotein and albumin were the important contributing factors in human serum. Various dilutions of 2.5% trypsin in phosphate-buffered saline specifically reduced background fluorescence under proper test conditions. By employing a trypsin digestion method, a semiautomated procedure utilizing a visual readout has been postulated as feasible.

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References

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