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. 1969 Mar;65(3):477-84.

Improvements in the preparation of heterologous antilymphocyte globulin with special reference to absorption and diethylaminoethyl cellulose batch production

N Kashiwagi, C G Groth, J R Amend, L Gecelter, H Blanchard, T E Starzl

Improvements in the preparation of heterologous antilymphocyte globulin with special reference to absorption and diethylaminoethyl cellulose batch production

N Kashiwagi et al. Surgery. 1969 Mar.
No abstract available

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Figures

Fig. 1
Fig. 1
Immunoelectrophoresis of immune horse serum (ALS) before and after absorption with human plasma (1 part to 10 parts horse serum), red cells, and thrombocytes. Above: Before absorption. Note the precipitin lines against most of the human plasma protein fractions including fibrinogen (arrow). Below: After absorption. All precipitin lines have disappeared except for a faint one in the alpha2 region.
Fig. 2
Fig. 2
Electrophoretic and immunoelectrophoretic patterns of unabsorbed antihuman ALS, the raw globulin obtained with a single ammonium sulfate precipitation of absorbed ALS, and the final product (pure gamma-G globulin) after subsequent DEAE-cellulose batch extraction.
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References

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