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. 1969 Mar;65(3):477-84.

Improvements in the preparation of heterologous antilymphocyte globulin with special reference to absorption and diethylaminoethyl cellulose batch production

N Kashiwagi, C G Groth, J R Amend, L Gecelter, H Blanchard, T E Starzl

Improvements in the preparation of heterologous antilymphocyte globulin with special reference to absorption and diethylaminoethyl cellulose batch production

N Kashiwagi et al. Surgery. 1969 Mar.
No abstract available

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Figures

Fig. 1
Fig. 1
Immunoelectrophoresis of immune horse serum (ALS) before and after absorption with human plasma (1 part to 10 parts horse serum), red cells, and thrombocytes. Above: Before absorption. Note the precipitin lines against most of the human plasma protein fractions including fibrinogen (arrow). Below: After absorption. All precipitin lines have disappeared except for a faint one in the alpha2 region.
Fig. 2
Fig. 2
Electrophoretic and immunoelectrophoretic patterns of unabsorbed antihuman ALS, the raw globulin obtained with a single ammonium sulfate precipitation of absorbed ALS, and the final product (pure gamma-G globulin) after subsequent DEAE-cellulose batch extraction.
See this image and copyright information in PMC

References

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    1. Iwasaki Y, Porter KA, Amend J, Marchioro TL, Zuhlke V, Starzl TE. The preparation and testing of horse antidog and antihuman antilymphoid plasma or serum and its protein fractions. Surg. Gynec. & Obst. 1967;124:1. - PMC - PubMed
    1. Kashiwagi N, Brantigan CO, Brettschneider L, Groth CG, Starzl TE. Clinical reactions and serologic changes following the administration of heterologous antilymphocyte globulin to human recipients of renal homografts. Ann. Int. Med. 1968;68:275. - PMC - PubMed

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