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. 1969 Aug;18(2):174-6.
doi: 10.1128/am.18.2.174-176.1969.

Detection of nuclease activity in semisolid and broth cultures

R V Lachica, R H Deibel

Detection of nuclease activity in semisolid and broth cultures

R V Lachica et al. Appl Microbiol. 1969 Aug.

Abstract

A method for the detection of deoxyribonuclease activity in semisolid agar cultures was investigated. When cultures were overlaid with an acridine orangedeoxyribonucleate-agar (ADA) mixture, incubated for 1 to 3 hr, and observed under ultraviolet light, clear halos developed around colonies that produced deoxyribonuclease. A variation of the method for use with broth cultures involved impregnation of filter-paper discs with a small portion of the culture and overlaying the discs with the ADA mixture. This alteration has the advantage that the test tube cultures are easily heat-treated prior to assay to determine the heat resistance of the enzyme.

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References

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