Brush border development in the intestinal absorptive cells of Xenopus during metamorphosis
- PMID: 4187705
- PMCID: PMC2107785
- DOI: 10.1083/jcb.44.1.151
Brush border development in the intestinal absorptive cells of Xenopus during metamorphosis
Abstract
The differentiation of the brush border which makes up the apical free surface of intestinal absorptive cells has been studied by electron microscopy. Specimens of Xenopus small intestine were fixed at various stages during metamorphosis, the time when a new intestinal epithelium forms. The interpretation of details described herein emphasizes the role of "surface-forming" vesicles. These vesicles are thought to provide membrane both for the initial expansion of the apical surface and for the later elongation of the microvilli. The latter are believed to be "molded" around filamentous cores that appear early in differentiation. The cores are attached to the apical membrane and extend vertically into the supranuclear cytoplasm. This interpretation rests chiefly on (a) the resemblance, both in morphology and in staining properties with colloidal thorium, between the membrane that limits the vesicles and that which limits the microvilli and (b) the distribution and time of appearance of the vesicles with respect to development of the microvilli. According to this view, the specific properties of surface membrane reside in preformed units that arise within the supranuclear cytoplasm. This morphogenetic process probably involves participation of the Golgi region as the site where the complex macromolecular architecture of the cell surface is assembled.
Similar articles
-
An electron microscopic study of the intestinal villus. II. The pathway of fat absorption.J Biophys Biochem Cytol. 1959 May 25;5(3):373-84. doi: 10.1083/jcb.5.3.373. J Biophys Biochem Cytol. 1959. PMID: 13664677 Free PMC article.
-
Acid mucopolysaccharide localization in intestinal absorptive cells after irradiation. BNWL-714.BNWL Rep. 1968 May:7.14-7+. BNWL Rep. 1968. PMID: 4186079 No abstract available.
-
Effects of puromycin on the structure of rat intestinal epithelial cells during fat absorption.J Cell Biol. 1972 Jan;52(1):15-40. doi: 10.1083/jcb.52.1.15. J Cell Biol. 1972. PMID: 4331298 Free PMC article.
-
The differentiation and redifferentiation of the intestinal epithelium and its brush border membrane.Ciba Found Symp. 1979 Jan 16-18;(70):31-50. doi: 10.1002/9780470720530.ch3. Ciba Found Symp. 1979. PMID: 396135 Review.
-
Presence of brush cells in the mouse gallbladder.Microsc Res Tech. 1997 Sep 15;38(6):598-608. doi: 10.1002/(SICI)1097-0029(19970915)38:6<598::AID-JEMT4>3.0.CO;2-B. Microsc Res Tech. 1997. PMID: 9330348 Review.
Cited by
-
Remodeling of the intestine during metamorphosis of Xenopus laevis.Proc Natl Acad Sci U S A. 2005 Mar 8;102(10):3720-5. doi: 10.1073/pnas.0409868102. Epub 2005 Feb 28. Proc Natl Acad Sci U S A. 2005. PMID: 15738398 Free PMC article.
-
Alterations in morphology of developing microvilli elicited by cytochalasin B. Studies of embryonic chick intestine in organ culture.J Cell Biol. 1974 Aug;62(2):566-74. doi: 10.1083/jcb.62.2.566. J Cell Biol. 1974. PMID: 4426920 Free PMC article. No abstract available.
-
Amphibian intestinal brush border enzymes during thyroxine-induced metamorphosis.Histochemistry. 1980;70(1):33-42. doi: 10.1007/BF00508844. Histochemistry. 1980. PMID: 6970191
-
Induction of metamorphosis by thyroid hormone in anuran small intestine cultured organotypically in vitro.In Vitro Cell Dev Biol. 1991 Nov;27A(11):853-7. doi: 10.1007/BF02630987. In Vitro Cell Dev Biol. 1991. PMID: 1748625
-
Association of the Golgi complex with the plasma membrane of amphibian embryonic cells.Protoplasma. 1973;76(1):115-22. doi: 10.1007/BF01279678. Protoplasma. 1973. PMID: 4365030 No abstract available.