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. 1969 Nov;100(2):1083-90.
doi: 10.1128/jb.100.2.1083-1090.1969.

Surface localization of Escherichia coli 5'-nucleotidase by electron microscopy

I Nisonson, M Tannenbaum, H C Neu

Surface localization of Escherichia coli 5'-nucleotidase by electron microscopy

I Nisonson et al. J Bacteriol. 1969 Nov.

Abstract

The 5'-nucleotidase of Escherichia coli was shown to be located at the cell wall surface by histochemical techniques utilizing the deposition of inorganic phosphate. Penetration of the 5'-nucleotidase in the periplasmic space was seen only in cells treated with ethylenediaminetetraacetic acid (EDTA)-tris(hydroxymethyl)aminomethane (Tris). The 3'-nucleotidase of E. coli was also found to have a surface location, and periplasmic precipitation of inorganic phosphate was seen only after EDTA-Tris-sucrose exposure.

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References

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