Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1970 Jan;65(1):122-8.
doi: 10.1073/pnas.65.1.122.

A general method for the specific staining of intracellular antigens with ferritin-antibody conjugates

J D McLean, S J Singer

A general method for the specific staining of intracellular antigens with ferritin-antibody conjugates

J D McLean et al. Proc Natl Acad Sci U S A. 1970 Jan.

Abstract

A general method is described whereby an intracellular macromolecule can be specifically stained with its antibody conjugated to ferritin. In this method cells or cell organelles are fixed, embedded in bovine serum albumin cross-linked with glutaraldehyde or formaldehyde, and then sectioned. This procedure preserves antigenic determinants as well as the cellular ultrastructure. The application of the ferritin-antibody conjugate to the section produces specific staining of the exposed antigen.

PubMed Disclaimer

References

    1. Virology. 1961 Jun;14:292-6 - PubMed
    1. J Cell Biol. 1967 Sep;34(3):757-71 - PubMed
    1. J Cell Biol. 1968 Apr;37(1):117-21 - PubMed
    1. J Cell Biol. 1964 Mar;20:518-21 - PubMed
    1. J Biophys Biochem Cytol. 1961 Mar;9:671-87 - PubMed

LinkOut - more resources