doi: 10.1042/bj1330037.
The purification and properties of extracellular glycosidases of the cellular slime mould Dictyostelium discoideum
- PMID: 4198517
- PMCID: PMC1177668
- DOI: 10.1042/bj1330037
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The purification and properties of extracellular glycosidases of the cellular slime mould Dictyostelium discoideum
Biochem J.
1973 May.
Abstract
The purification of beta-N-acetylhexosaminidase, alpha-glucosidase, alpha-mannosidase and beta-glucosidase from the spent growth medium of Dictyostelium discoideum strain Ax-2 myxamoebae is described. beta-N-Acetylhexosaminidase and alpha-glucosidase were obtained in high yield and as homogeneous preparations whereas the alpha-mannosidase preparation consisted of two electrophoretically distinct isoenzymes. The physical, chemical and kinetic properties of these enzymes are described.
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