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. 1973 May;133(1):37-47.
doi: 10.1042/bj1330037.

The purification and properties of extracellular glycosidases of the cellular slime mould Dictyostelium discoideum

D Every, J M Ashworth

The purification and properties of extracellular glycosidases of the cellular slime mould Dictyostelium discoideum

D Every et al. Biochem J. 1973 May.

Abstract

The purification of beta-N-acetylhexosaminidase, alpha-glucosidase, alpha-mannosidase and beta-glucosidase from the spent growth medium of Dictyostelium discoideum strain Ax-2 myxamoebae is described. beta-N-Acetylhexosaminidase and alpha-glucosidase were obtained in high yield and as homogeneous preparations whereas the alpha-mannosidase preparation consisted of two electrophoretically distinct isoenzymes. The physical, chemical and kinetic properties of these enzymes are described.

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References

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