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. 1973 Oct;70(10):2997-3001.
doi: 10.1073/pnas.70.10.2997.

Relation of detergent HLB number to solubilization and stabilization of D-alanine carboxypeptidase from Bacillus subtilis membranes

J N Umbreit, J L Strominger

Relation of detergent HLB number to solubilization and stabilization of D-alanine carboxypeptidase from Bacillus subtilis membranes

J N Umbreit et al. Proc Natl Acad Sci U S A. 1973 Oct.

Abstract

The ability of various nonionic detergents to solubilize D-alanine carboxypeptidase and other membrane-bound enzymes was correlated to a physical property of the detergent, the HLB number. Only a fairly narrow range of detergents were effective solubilizing agents. Purified carboxypeptidase required either a detergent or detergent plus a lipid fraction for stability. Only those detergents effective in solubilizing the enzyme were effective in stabilizing it.

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References

    1. Virology. 1969 Nov;39(3):408-18 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1972-4 - PubMed
    1. Can J Biochem Physiol. 1959 Aug;37(8):911-7 - PubMed

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