Phenotype recognition of pyocyanine mutants in pseudomonas aeruginosa
- PMID: 4202997
- PMCID: PMC246558
- DOI: 10.1128/jb.117.1.312-314.1974
Phenotype recognition of pyocyanine mutants in pseudomonas aeruginosa
Abstract
Certain classes of pyocyanine mutants in Pseudomonas aeruginosa escape detection when screened in the presence of wild-type or other mutant cells. A technique is described for recognizing mutant phenotypes after cells are in individual agar wells. The procedure eliminates cross-feeding phenomena as well as the masking of mutant phenotypes by diffusing pyocyanine produced by nearby clones.
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