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. 1974 Jan;71(1):40-4.
doi: 10.1073/pnas.71.1.40.

Calf tendon procollagen peptidase: its purification and endopeptidase mode of action

L D Kohn, C Isersky, J Zupnik, A Lenaers, G Lee, C M Lapiére

Calf tendon procollagen peptidase: its purification and endopeptidase mode of action

L D Kohn et al. Proc Natl Acad Sci U S A. 1974 Jan.

Abstract

The procollagen peptidase activity of calf tendon has been purified. The enzyme has a high degree of specificity for native procollagen and converts both pro alpha1 and alpha2, to alpha1 and alpha2, respectively. The purified enzyme is an endopeptidase which excises the amino terminal peptide extensions of the precursor chains in block; the molecular size and amino-acid composition of the excised peptides compare favorably with those predicted in previous reports. Antisera to the enzyme and to procollagen have been prepared and have been used to characterize the enzyme, the enzymatically excised peptides, and the enzyme-peptide complex in reaction mixtures.

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References

    1. Science. 1972 Feb 4;175(4021):544-6 - PubMed
    1. Ann N Y Acad Sci. 1964 Dec 28;121:404-27 - PubMed
    1. FEBS Lett. 1972 Dec 1;28(2):247-250 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2727-31 - PubMed
    1. Anal Biochem. 1967 Jul;20(1):150-4 - PubMed

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