Time course of purine nucleoside phosphorylase occurrence in sporulation of Bacillus cereus
- PMID: 4204910
- PMCID: PMC251236
- DOI: 10.1128/jb.111.1.33-36.1972
Time course of purine nucleoside phosphorylase occurrence in sporulation of Bacillus cereus
Abstract
Purine nucleoside phosphorylase (EC 2.4.2.1) from Bacillus cereus T was examined at hourly intervals during growth and sporulation. The enzyme has maximal activity in extracts prepared from cells during stages I and II. The activity during exponential growth is only 6.6% of the maximum and that in free spores is only 3.3%. Conservation of the purine nucleoside phosphorylase during sporulation is apparent as shown by the gradual increase in heat resistance.
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