Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1974 May;118(2):329-33.
doi: 10.1128/jb.118.2.329-333.1974.

Localization of proteinase(s) near the cell surface of Streptococcus lactis

T D Thomas, B D Jarvis, N A Skipper

Localization of proteinase(s) near the cell surface of Streptococcus lactis

T D Thomas et al. J Bacteriol. 1974 May.

Abstract

Two criteria suggest that most of the proteinase of Streptococcus lactis is localized in the cell wall. (i) Intact cells possess proteinase activity when incubated with a high-molecular-weight substrate. (ii) Most of the cell-bound proteinase activity is released during spheroplast formation under conditions which result in the release of only 1% of the intracellular enzymes aldolase and glyceraldehyde-3-phosphate dehydrogenase. The solubilized cell wall, plasma membrane, and cytoplasm fractions contained 84, 0, and 16%, respectively, of the total proteinase activity with casein as substrate. The physiological role of a surface-bound proteinase in this organism is discussed.

PubMed Disclaimer

References

    1. Appl Microbiol. 1967 Jul;15(4):851-6 - PubMed
    1. Biochemistry. 1970 Feb 17;9(4):786-95 - PubMed
    1. Biochim Biophys Acta. 1972 Aug 31;278(1):147-54 - PubMed
    1. J Bacteriol. 1962 Mar;83:697-8 - PubMed
    1. Biochim Biophys Acta. 1965 Oct 18;107(3):539-45 - PubMed

MeSH terms