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. 1974 May;118(2):654-62.
doi: 10.1128/jb.118.2.654-662.1974.

Detachment and chemical characterization of the regularly arranged subunits from the surface of an Acinetobacter

M J Thornley, K J Thorne, A M Glauert

Detachment and chemical characterization of the regularly arranged subunits from the surface of an Acinetobacter

M J Thornley et al. J Bacteriol. 1974 May.

Abstract

Acinetobacter sp. strain MJT/F5/199A carries an array of tetragonally arranged subunits on its outer surface. The subunits can be detached from isolated cell walls by incubation with 1 M urea or by washing with water after treatment with 10 mM ethylenediaminetetraacetic acid or ethyleneglycol-bis(beta-aminoethylether)N,N'-tetraacetic acid. After removal of the urea, they reaggregate into the same ordered array at air-water interfaces in the presence of MgCl(2). The detached subunits were characterized as an acidic protein of molecular weight 65,000. They represent one-fifth of the total cell wall protein.

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