The use of immunoadsorbent columns for the isolation of antibodies specific for antigens associated with human bronchogenic carcinoma
- PMID: 4208459
- PMCID: PMC2009101
- DOI: 10.1038/bjc.1974.57
The use of immunoadsorbent columns for the isolation of antibodies specific for antigens associated with human bronchogenic carcinoma
Abstract
An immunoadsorbent technique is described whereby tumour-specific antibodies may be isolated. Extracts from normal human lung tissue were pooled and bound to cyanogen bromide activated Sepharose 4B. Antisera raised in rabbits to a variety of extracts from human bronchogenic carcinoma were passed through these immunoadsorbent columns to yield antisera specific for tumour-associated antigens as demonstrated by immunodiffusion and immunoelectrophoresis.
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