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. 1974 Mar;29(3):183-8.
doi: 10.1038/bjc.1974.57.

The use of immunoadsorbent columns for the isolation of antibodies specific for antigens associated with human bronchogenic carcinoma

R D Watson, A G Smith, J G Levy
Free PMC article

The use of immunoadsorbent columns for the isolation of antibodies specific for antigens associated with human bronchogenic carcinoma

R D Watson et al. Br J Cancer. 1974 Mar.
Free PMC article

Abstract

An immunoadsorbent technique is described whereby tumour-specific antibodies may be isolated. Extracts from normal human lung tissue were pooled and bound to cyanogen bromide activated Sepharose 4B. Antisera raised in rabbits to a variety of extracts from human bronchogenic carcinoma were passed through these immunoadsorbent columns to yield antisera specific for tumour-associated antigens as demonstrated by immunodiffusion and immunoelectrophoresis.

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