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Comparative Study
. 1975 Nov;2(5):453-5.
doi: 10.1128/jcm.2.5.453-455.1975.

Methods to improve detection of pneumococci in respiratory secretions

J A Dilworth, P Stewart, J M Gwaltney Jr, J O Hendley, M A Sande
Comparative Study

Methods to improve detection of pneumococci in respiratory secretions

J A Dilworth et al. J Clin Microbiol. 1975 Nov.

Abstract

Simple methods to enhance the detection of pneumococci in respiratory secretions are needed. Sheep blood agar containing 5 mug of gentamicin per ml was more often positive (89%) than either standard sheep blood agar (54%) or mouse inoculation (65%) in recovering pneumococci from 62 adult and pediatric patients. In adults, the direct quellung test on sputum smear was a rapid, sensitive method for predicting subsequent pneumococcal isolation by culture (19 of 20 patients, 95%). The quellung test and gentamicin plate show improved sensitivity over current techniques for pneumococcal detection and can be recommended for general use.

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