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. 1966 Feb;98(2):378-88.
doi: 10.1042/bj0980378.

Oxidative phosphorylation accompanying oxidation of short-chain fatty acids by rat-liver mitochondria

F J Hird, M J Weidemann

Oxidative phosphorylation accompanying oxidation of short-chain fatty acids by rat-liver mitochondria

F J Hird et al. Biochem J. 1966 Feb.

Abstract

1. The factors concerned in the estimation of P/O ratios when fatty acids are oxidized by rat-liver mitochondria have been assessed. 2. The oxidation of butyrate, hexanoate and octanoate is accompanied by ATP synthesis. At low concentrations of the fatty acids, P/O ratios approximately 2.5 are obtained. 3. Oxidative phosphorylation is uncoupled, respiratory control ratios are lowered and respiration is inhibited when the concentration of the fatty acid in the incubating medium is raised (to 5-10mm); octanoate is a more potent uncoupler than either hexanoate or butyrate. 4. Serum albumin and carnitine, either singly or in combination, protect the mitochondria from the effect exerted by the fatty acids. 5. The rate of oxidation of short-chain fatty acids in the presence of ADP is increased in the presence of carnitine.

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References

    1. Biochim Biophys Acta. 1956 Sep;21(3):458-66 - PubMed
    1. Biochim Biophys Acta. 1959 Oct;35:422-34 - PubMed
    1. J Biol Chem. 1964 Jan;239:339-44 - PubMed
    1. Proc Soc Exp Biol Med. 1962 Jan;109:215-8 - PubMed
    1. J Biol Chem. 1953 Sep;204(1):435-44 - PubMed