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. 1979 Sep 1;181(3):759-61.
doi: 10.1042/bj1810759.

Dodecyl sulphate/polyacrylamide-gel electrophoresis at low pH values and low temperatures

R Lichtner, H U Wolf

Dodecyl sulphate/polyacrylamide-gel electrophoresis at low pH values and low temperatures

R Lichtner et al. Biochem J. .

Abstract

A simple method is described for dodecyl sulphate/polyacrylamide-gel electrophoresis of pH- and temperature-labile biological intermediates. The method is based on a catalyst system that works at temperatures of 2--4 degrees C and pH values of 2--4 and an appropriate buffer system containing Li+ or Tris [CH2OH--C(CH2OH)2--NH3+] instead of Na+. This system does not lead to the precipitation of 1% dodecyl sulphate.

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References

    1. Anal Biochem. 1965 May;11(2):211-8 - PubMed
    1. J Biol Chem. 1969 Aug 25;244(16):4406-12 - PubMed
    1. Anal Biochem. 1969 Feb;27(2):205-11 - PubMed
    1. Anal Biochem. 1965 Sep;12(3):526-41 - PubMed
    1. Anal Biochem. 1965 Nov;13(2):336-44 - PubMed