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. 1969 Jan;3(1):33-7.
doi: 10.1128/JVI.3.1.33-37.1969.

Transfection of lysostaphin-treated cells of Staphylococcus aureus

H G Riggs Jr, E D Rosenblum

Transfection of lysostaphin-treated cells of Staphylococcus aureus

H G Riggs Jr et al. J Virol. 1969 Jan.

Abstract

After treatment with 1 unit of lysostaphin per ml for 3 min, two strains of Staphylococcus aureus, 233 and PS 44A HJD, were transfected with phenol-extracted deoxyribonucleic acid (DNA) from the staphylococcal bacteriophages, 53 and 44A HJD, respectively. The number of transfected cells was low in both systems, approximately two in 10(7) enzyme-treated cells. There was a saturation effect at high concentrations of DNA; optimal results were obtained at concentrations between 10 to 25 mug/ml. Growth curves and fluctuation tests indicated that cells of strain 44A HJD infected with phage, then converted to protoplasts by a 10-min treatment with lysostaphin, produce only one phage particle and lose their ability to lyse spontaneously in hypertonic media.

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References

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