The 3' noncoding region of beta-globin mRNA is not essential for in vitro translation
- PMID: 424286
- PMCID: PMC327679
- DOI: 10.1093/nar/6.1.153
The 3' noncoding region of beta-globin mRNA is not essential for in vitro translation
Abstract
Rabbit beta globin DNA sequence, excised from plasmid pbetaG1, directs in vitro synthesis of beta-globin in a transcription-translation cell-free system, even after specific elimination of the entire 3'-noncoding region. A DNA restriction fragment carrying this 3' noncoding region and hybridized to globin mRNA cannot arrest the cell-free translation of beta-globin mRNA.
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