doi: 10.1093/nar/6.2.689.
A new procedure for purifying histone pairs H2A + H2B and H3 + H4 from chromatin using hydroxylapatite
- PMID: 424310
- PMCID: PMC327721
- DOI: 10.1093/nar/6.2.689
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A new procedure for purifying histone pairs H2A + H2B and H3 + H4 from chromatin using hydroxylapatite
Nucleic Acids Res.
1979 Feb.
Free PMC article
Abstract
A method to purify histone groups H2A+H2B and H3+H4 using dissociation with NaCl and hydroxylapatite chromatography is presented. The procedure is simple, involves mild solvents, and provides milligram quantities of histones of high purity. The histone pairs prepared by this method can regenerate chromatin-like characteristics when combined and reconstituted with DNA.
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