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. 1979 Mar 9;203(4384):1019-21.
doi: 10.1126/science.424726.

Methylation of mouse liver DNA studied by means of the restriction enzymes msp I and hpa II

J Singer, J Roberts-Ems, A D Riggs

Methylation of mouse liver DNA studied by means of the restriction enzymes msp I and hpa II

J Singer et al. Science. .

Abstract

The restriction enzymes Hpa II and Msp I both recognize the sequence 5'-CCGG (C, cytosine; G, guanine). However, Hpa II cuts mouse liver DNA to fragments four times larger than does Msp I. The size of DNA cut by Msp I is close to that predicted from base composition and nearest neighbor analysis. The most probable explanation of these results is that in mouse the site 5'-CCGG is highly methylated.

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