Mucopolysaccharide which regulates growth in Neurospora

Abstract

Neurospora produces a mucopolysaccharide (called MP) which inhibits its growth, causes vacuolation and agglutination of its cells, and precipitates its purified membrane protein. Cultures of a colonial strain display a phase of slow growth; the induction of this phase is traced to the production of MP by the mold. Stationary-phase cultures of wild type also produce MP. MP is a polymer of galactosamine, its amino groups only partially acetylated, probably containing other minor components. MP molecular weight is approximately 10(6). Complete acetylation abolishes the biological activities of MP. It is suggested that the regulatory effect of MP is mediated by its interaction with the protoplasmic membrane.