Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1971 Aug;68(8):1824-7.
doi: 10.1073/pnas.68.8.1824.

Stimulation of RNA transcription from pea and corn DNA by protein retained on sepharose coupled to 2,4-dichlorophenoxyacetic acid

M A Venis

Stimulation of RNA transcription from pea and corn DNA by protein retained on sepharose coupled to 2,4-dichlorophenoxyacetic acid

M A Venis. Proc Natl Acad Sci U S A. 1971 Aug.

Abstract

When crude extracts from pea or corn shoots are passed through a column of agarose coupled to a derivative of the synthetic plant hormone 2,4-dichlorophenoxyacetic acid, certain protein fractions are retained, whereas unmodified agarose adsorbs no protein. Appropriate elution yields a protein factor that stimulates DNA-dependent RNA synthesis (supported by Escherichia coli polymerase) by 40-200%. Preliminary evidence suggests that the factor may be influencing initiation of RNA chains.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1965 May;53(5):1104-11 - PubMed
    1. Biochemistry. 1966 Aug;5(8):2563-70 - PubMed
    1. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1740-3 - PubMed
    1. Biochim Biophys Acta. 1968 Mar 18;157(1):218-9 - PubMed
    1. Biochemistry. 1968 Feb;7(2):601-5 - PubMed

MeSH terms

LinkOut - more resources