The polarity of the proximal tubule cell in rat kidney. Different surface charges for the brush-border microvilli and plasma membranes from the basal infoldings
- PMID: 4261147
- PMCID: PMC2108866
- DOI: 10.1083/jcb.54.2.232
The polarity of the proximal tubule cell in rat kidney. Different surface charges for the brush-border microvilli and plasma membranes from the basal infoldings
Abstract
Two different membrane fractions were obtained from a brush-border fraction of rat kidney cortex by using their different electrical surface charges in preparative free-flow electrophoresis. One membrane fraction contained only morphologically intact microvilli and was characterized by a high specific activity of alkaline phosphatase. The other fraction morphologically resembled classical plasma membranes by possessing junctional complexes and a high Na-K-ATPase activity The contamination of the isolated membrane fractions by other cell organelles was extremely low These two fractions represent the apical (luminal) and the basal (interstitial) area of the renal proximal tubule cell membrane and clearly demonstrate the polarity of this cell.
Similar articles
-
Further evidence for the existence of an intrinsic bicarbonate-stimulated Mg2+-ATPase in brush border membranes isolated from rat kidney cortex.J Membr Biol. 1979 Sep;49(3):235-51. doi: 10.1007/BF01871120. J Membr Biol. 1979. PMID: 226712
-
[Membrane function of the kidney].Bull Schweiz Akad Med Wiss. 1976 Dec;32(4-6):251-76. Bull Schweiz Akad Med Wiss. 1976. PMID: 137758 German.
-
Isolation of the basal and lateral plasma membranes of rat kidney tubule cells.Biochim Biophys Acta. 1976 May 21;433(3):531-46. doi: 10.1016/0005-2736(76)90279-0. Biochim Biophys Acta. 1976. PMID: 132191
-
Electrophysiology of plasma membrane vesicles.Am J Physiol. 1984 Apr;246(4 Pt 2):F363-72. doi: 10.1152/ajprenal.1984.246.4.F363. Am J Physiol. 1984. PMID: 6372509 Review.
-
Molecular basis of transepithelial transport in the proximal tubule of rat kidney: properties of the luminal and the contraluminal cell membrane.Fortschr Zool. 1975;23(2-3):261-78. Fortschr Zool. 1975. PMID: 174985 Review. No abstract available.
Cited by
-
Transport of p-aminohippuric acid by plasma membrane vesicles isolated from rat kidney cortex.Pflugers Arch. 1976 Feb 24;361(3):269-77. doi: 10.1007/BF00587292. Pflugers Arch. 1976. PMID: 943770
-
Phenomenologic description of Na+, Cl- and HCO-3 absorption from proximal tubules of rat kidney.Pflugers Arch. 1973 Oct 22;343(3):189-220. doi: 10.1007/BF00586045. Pflugers Arch. 1973. PMID: 4798991 No abstract available.
-
The Feldberg Lecture 1976. Solute transport across epithelia: what can we learn from micropuncture studies in kidney tubules?J Physiol. 1979 Mar;288:1-31. J Physiol. 1979. PMID: 381634 Free PMC article. Review. No abstract available.
-
Characterization of the fetal glucose transporter in rabbit kidney. Comparison with the adult brush border electrogenic Na+-glucose symporter.J Clin Invest. 1988 Aug;82(2):379-87. doi: 10.1172/JCI113609. J Clin Invest. 1988. PMID: 3403709 Free PMC article.
-
Enzyme changes in experimental renal microcystic disease.Br J Exp Pathol. 1973 Oct;54(5):555-65. Br J Exp Pathol. 1973. PMID: 4271322 Free PMC article.
