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. 1973 Mar;24(3):435-43.

Biological and physicochemical properties of purified anti-DNP guinea-pig non-precipitating antibodies

R A Margni, S Hajos

Biological and physicochemical properties of purified anti-DNP guinea-pig non-precipitating antibodies

R A Margni et al. Immunology. 1973 Mar.

Abstract

Methods for isolation and purification of precipitating and non-precipitating guinea-pig antibodies are described.

The physicochemical properties of γ1 and γ2 non-precipitating antibodies are similar to γ1 and γ2 precipitating ones. Biological properties are also similar excepting the reverse Arthus reaction, which is positive with the precipitating and negative with the non-precipitating antibodies.

Bivalence of these antibodies was experimentally demonstrated. Precipitating antibodies K0 do not differ greatly from those obtained with the corresponding non-precipitating ones. The incapacity to precipitates with the antigen may be a consequence of a steric impediment.

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