doi: 10.1084/jem.138.1.324.
Proliferation of B and T cells in mixed lymphocyte cultures
- PMID: 4268630
- PMCID: PMC2180546
- DOI: 10.1084/jem.138.1.324
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Proliferation of B and T cells in mixed lymphocyte cultures
J Exp Med.
.
Abstract
Electrophoretically fractionated CBA/Ca spleen T cells alone respond to allogeneic cells in one-way MLC and to PHA. They do not respond to E. coli LPS. B cells alone do not respond to allogeneic cells nor to PHA, but do respond to LPS. When karyotypically distinguishable syngeneic mixtures of T and B lymphocytes are stimulated with allogeneic cells, at the most 5% of mitoses on 5-9th culture day are of B cell origin. This indicates that B cells are not substantially recruited to proliferate in the MLC.
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