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. 1979 Feb 20;551(1):1-9.
doi: 10.1016/0005-2736(79)90348-1.

Calcium-induced erythrocyte membrane changes. The role of adsorption of cytosol proteins and proteases

D W Allen, S Cadman

Calcium-induced erythrocyte membrane changes. The role of adsorption of cytosol proteins and proteases

D W Allen et al. Biochim Biophys Acta. .

Abstract

Changes in the membranes of human red cells similar to those of certain hemolytic anemias were produced by calcium in three model systems and found to result from membrane adsorption of cytosol proteins and from proteolysis. Proteins of the cytosol adsorbed to human erythrocyte membranes in the presence of calcium and extractable by EDTA were compared to those of the total cytosol by polyacrylamide gel electrophoresis and by isoelectric focusing. Catalase (EC 1.11.1.6) and band 8 were adsorbed to the membranes from the supernatant cytosol with calcium. Band 8 was a normal constitutent of the cytosol, apparently a single chain of molecular weight 24,000 with a pI of 5.35. Other calcium-induced membrane changes could be demonstrated to be due to cytosol protease(s) adsorbed to the membrane in the presence of calcium and extractable with EDTA. When membranes were incubated with the proteases and calcium the decrease in bands 1,2,3 and 4.1 and the appearance of multiple low molecular weight peptides typical of calcium-induced membrane effects resulted.

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