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. 1979 Mar 21;5(1):43-53.
doi: 10.1007/BF00535772.

Interaction of bovine rhodopsin with calcium ions. II: calcium release in bovine rod outer segments upon bleaching

G Nöll, H Stieve, J Winterhager

Interaction of bovine rhodopsin with calcium ions. II: calcium release in bovine rod outer segments upon bleaching

G Nöll et al. Biophys Struct Mech. .

Abstract

The calcium content of bovine rod outer segment (ROS) suspensions was determined by flame spectrophotometry to be about 0.2 Ca2+ per molecule rhodopsin. After bleaching of rhodopsin, a release of 0.01--0.1 Ca2+ per molecule rhodopsin from ROS into the solution was observed. These figures agree with some data in the literature (Appendix). A measured absorption increase of the Ca2+-indicator phthalein purple (10 degrees C, 562 nm, pH 9.3) occurs apparently simultaneously with the formation of metarhodopsin ii in ROS. This indicates that a light induced Ca2+-release of 12 calcium ions per photoactivated rhodopsin is coupled in time with the formation of metarhodopsin II.

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