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. 1973 Nov;70(11):3120-4.
doi: 10.1073/pnas.70.11.3120.

Studies on in vitro DNA synthesis. Isolation of DNA B gene product from Escherichia coli

M Wright, S Wickner, J Hurwitz

Studies on in vitro DNA synthesis. Isolation of DNA B gene product from Escherichia coli

M Wright et al. Proc Natl Acad Sci U S A. 1973 Nov.

Abstract

Formation of duplex DNA from varphiX174 single-stranded DNA by extracts of E. coli was previously shown to require the gene product of dna B. Using as an assay the stimulation of varphiX174 DNA-dependent synthesis in inactivated extracts of dna B temperature-sensitive cells, we purified the dna B gene product from wild-type E. coli as well as from a dna B temperature-sensitive mutant. The dna B temperature-sensitive gene product is more thermolabile than its wild-type counterpart.

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References

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