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. 1965 Nov;13(6):1026-9.
doi: 10.1128/am.13.6.1026-1029.1965.

Titration and neutralization of poliovirus in micro tissue culture under increased carbon dioxide

M Kende, M L Robbins

Titration and neutralization of poliovirus in micro tissue culture under increased carbon dioxide

M Kende et al. Appl Microbiol. 1965 Nov.

Abstract

Modification of the micro tissue culture technique, including incubation under increased CO(2), resulted in prolongation of the viability of the cells. As a consequence, satisfactory titrations of poliovirus and of poliovirus antiserum have been achieved by the micro method. The technique offers a number of advantages over conventional methods.

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