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. 1967 Jan;31(1):7-11.

African swine fever. II. Detection of the virus in swine tissues by means of the modified direct complement-fixation test

P Boulanger, G L Bannister, D P Gray, G M Ruckerbauer, N G Willis

African swine fever. II. Detection of the virus in swine tissues by means of the modified direct complement-fixation test

P Boulanger et al. Can J Comp Med Vet Sci. 1967 Jan.

Abstract

The modified direct complement-fixation test, supplemented with unheated normal calf serum, was used to demonstrate antibodies in sera of swine immunized to African swine fever virus. These antibodies did not react in the ordinary direct non-supplemented complement-fixation test.African swine fever complement-fixing antigen in infected swine tissue is not denatured by extraction with fat solvents. Consequently, good antigens devoid of non-specific reactivity were obtained by extraction with a mixture of acetone and ether. The virus was detected in infected swine tissue harvested one day after beginning of pyrexia. The modified direct complement-fixation test demonstrated cross-reactions between the six strains of virus studied.

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References

    1. Can J Comp Med Vet Sci. 1947 Aug;11(8):236-45 - PubMed
    1. Can J Comp Med Vet Sci. 1967 Jan;31(1):2-6 - PubMed
    1. Can J Comp Med Vet Sci. 1960 Sep;24(9):262-9 - PubMed
    1. Can J Comp Med Vet Sci. 1961 Jan;25(1):8-12 - PubMed
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